Medical Lab Technologist Macq's
Chapter 6
Sterilization, Laboratory Principal, Quality Assessment and Safety
Q1= Which of the following is not a characteristic of UV radiation?
a. nonionizing
b. high degree of penetration
c. forms thymine dimers in DNA chains
d. germicidal at 260 nm
e. carcinogenic
ANS= B
Q2= Which of the following can kill prions?
a. normal autoclaving
b. antibiotics
c. incineration
d. phenolics
e. alcohol
ANS= C
Q3= Which of these chemical agents control microbial growth via oxidation?
a. alcohols
b. heavy metals
c. per-oxygens
d. soaps
e. organic acids
ANS= C
Q4=. Which of the following is not suitable for sterilization in a steam autoclave?
a. stainless steel forceps
b. pipette tips
c. nutrient agar
d. enzyme solution
e. biohazard waste
ANS= D
5. Which of the following chemical agents is not used in the food processing industry?
a. aldehydes
b. organic acids
c. nitrates
d. terpenes
e. hydrogen peroxide
ANS= A
Q6=which process is used to destroy or eliminate viable microorganism that may be present in or on a particular product or package
A- bacteriostatic
B- sterilization
C- aseptic
D- bactericide
ANS= B
Q7= Hexachlorobenzene is a type of
a) POPs
b) DDT
c) Pollutants
d) Herbicides
Ans- A
(Persistent Organic Pollutants )
Q8=How is distilled water prepared?
A : Passing it through an exchange resin
B : Boiling and cooling
C : Passing through a cation exchange resin
D : Electrolysis of water
Ans- B
Q9= Which is the durable material used in heat resistant glassware?
A : Polyethylene
B : Sodalime
C : Polystyrene
D : Borosilicate
ANS- D
Q10= Which one of the following is the principle of an autoclave?
A : Direct heat
B : Hot steam
C : Hot pressure
D : Steam under pressure
ANS- D
Q11=Which one of the following is used for fumigation in microbiology laboratory?
A : Carbon dioxide
B : Formaldehyde
C : Ethylene Oxide
D : Phenols
ANS- B
Q12= Which of the following is an aluminasilicate glass that is at least six times stronger than borosilicate and is resistant to alkaline etching and scratching?
A. Kimax
B. Pyrex
C. Corning boron free
D. Corex
ANS=D
Q13=. The National Institute of Standards and Technology (NIST) requires that volumetric pipettes and flasks be certified as
A. Class A
B. Class B
C. Class C
D. Class D
ANS=A
Q14=. Which of the following desiccants is the most hygroscopic?
A. Silica gel
B. Alumina
C. Barium oxide
D. Magnesium perchlorate
ANS=D
Q15=. Lavender top blood collection tubes are used for complete blood counts. What is the anticoagulant in these tubes?
A. EDTA
B. Heparin
C. Sodium citrate
D. Sodium oxalate
ANS=A
Q16= SI units are the designated units employed by the International System of Units. The unit class that encompasses the seven fundamental quantities of measurement is
A. Base
B. Primary
C. Derived
D. Elemental
ANS=A
Q17=. Which of the following parameters does not have an effect on centrifugal force?
A. Temperature of the centrifuge
B. Mass of the material being centrifuged
C. Speed of rotation
D. Radius of the centrifuge
ANS=A
Q18= What class of weights should be used to check the calibration of analytical balances?
A. J
B. M
C. P
D. S
ANS=D
Q19= "To deliver" (TD) pipettes are identified by
A. Two etched bands near the top
B. Self-draining capacity
C. Dual-purpose pipette labels
D. Blue graduation levels
ANS=A
Q20= If a laboratory needs to keep certain chemical materials dry, the apparatus used will be a
A. Buret
B. Desiccator
C. Separatory funnel
D. Vacuum
ANS=B
Q21 = Which of the following is an advantage of the angle-head centrifuge over the horizontal-head centrifuge?
A. Less air friction
B. Smaller increase in sample temperature during centrifugation
C. Can be operated at a higher speed
D. All the above
ANS=D
Q22=. The type of water desired for use in test methods requiring maximum accuracy and precision is
A. Distilled
B. Pure grade
C. Reagent grade
D. Special reagent water
ANS=C
Q23= The speed of a centrifuge should be checked at least once every 3 months with a(n)
A. Tachometer
B. Wiper
C. Potentiometer
D. Ergometer
ANS=A
Q24= The type of balance that uses an electromagnetic force to counterbalance the load placed on the pan is a(n)
A. Trip balance
B. Class A balance
C. Class S balance
D. Electronic balance
ANS=D
Q25= Which of the following blood collection tube tops would contain a serum separator gel?
A. Blue
B. Grey
C. Lavender
D. Speckled
ANS=D
Q26= When performing a venipuncture, which of the following is the proper order of draw for evacuated blood collection tubes?
A. Sterile specimen, light blue top, and plain red top
B. Light blue top, plain red top, and sterile specimen
C. Lavender top, light blue top, plain red top
D. Green top, sterile specimen, and plain red top
ANS=A
Laboratory Quality Assessment
Q27= What term applies to the sum of all the values in a set of numbers divided by the number of values in that set?
A. Median
B. Mode
C. Arithmetic mean
D. Geometric mean
ANS=C
Q28= Calculate the coefficient of variation (percent) for a set of data where the mean (x) = 89 mg/dL and 2 standard deviations 0) = 14 mg/dL.
A. 7.8
B. 7.9
C. 15.7
D. 15.8
ANS=B
Q29= Which of the following parameters is not needed to determine the predictive value of a positive test?
A. Sensitivity
B. Specificity
C. Disease prevalence
D. Precision
ANS=D
Q30= Which of the following must be known in order to determine the sensitivity of a test?
A. True positives and false negatives
B. True negatives and false positives
C. True positives and false positives
D. True negatives and false negatives
ANS=A
Q31= A new test to assess for the presence of malignancy has been developed. By testing a group of benign individuals, it is determined that 45 of 50 subjects test negative for the new marker. What is the specificity of this new assay?
A. 10%
B. 11%
C. 90%
D. 100%
ANS=C
Q32= Which of the following must be known in order to determine the predictive value of a negative test; that is, the percentage of individuals who test negative and are not diseased?
A. True negatives and false negatives
B. True positives and false positives
C. True positives and false negatives
D. True negatives and false positives
ANS=A
Laboratory Safety
Q33= Which of the following is a reactive chemical that has the potential to become shock sensitive if stored for a prolonged period of time?
A. Xylene
B. Picric acid
C. Chloroform
D. Phenol
ANS=B
Q34= A fire extinguisher used in the event of an electrical fire should include which of the following classifications?
A. Type A
B. Type B
C. Type C
D. Type D
ANS=C
Q35= In the National Fire Protection Association identification system, four color-coded, diamond-shaped symbols are arranged to form a larger diamond shape. What type of hazard does the blue diamond identify?
A. Flammable
B. Health
C. Reactivity
D. Contact
ANS=B
Q36= Xylene, ethanol, methanol, and acetone would be in which hazard class?
A. Corrosive
B. Flammable
C. Oxidizer
D. Carcinogen
ANS=B
Q37= Which of the following terms is used to identify a chemical that causes cancer?
A. Mutagen
B. Teratogen
C. Carcinogen
D. Reactive
ANS=C
Q38= The maximum chemical exposure allowable for an employee during one 8-hour day is the
A. Flashpoint
B. HEPA standard
C. Threshold limit value
D. Limit established by the United Nations
ANS=C
Q39= The air from a chemical fume hood is
A. Recirculated through a HEPA filter
B. Totally exhausted from the building
C. Totally exhausted from the building through a HEPA filter
D. Recirculated through a HEPA filter (30%) and 70% is exhausted to the outside
ANS=B
Q40=The air-handling system for a microbiology laboratory should
A. Maintain negative pressure with respect to the administrative areas
B. Maintain positive pressure with respect to the administrative areas
C. Have no particular requirement
D. Have a HEPA filter
ANS=A
Q41= The major job-related disease hazard in clinical laboratories is
A. Tularemia
B. Salmonella
C. Tuberculosis
D. Hepatitis
ANS=D
Q42= What written plan of specific measures must laboratories have in place to minimize the risk of exposure to bloodborne pathogens?
A. Chemical hygiene plan
B. Exposure control plan
C. Material safety data sheets
D. Infection control plan
ANS=B
Q43= Which of the following information is not found on a material safety data sheet?
A. Health hazard data
B. Fire and explosion hazard data
C. First aid measures
D. Cost of chemical
ANS=D
Q44= Work is being done with Mycobacterium tuberculosis in the microbiology laboratory. It is important that you enter this laboratory while work is being done with positive samples. What is the most important personal protective equipment you should don before entering this laboratory?
A. Carbon cartridge respirator
B. Mask
C. Gloves
D. N95 HEPA filter respirator
ANS=D
Q45= When working with chemicals, the selection of your gloves depends on
A. The chemical hazard and the tasks involved
B. How far you must transport the chemical
C. Whether or not you use a chemical fume hood
D. Whatever is available in the laboratory
ANS=A
Q46= What is the maximum capacity of a chemical storage safety can used in the laboratory?
A. 1 gallon
B. 5 gallons
C. 20 gallons
D. 55 gallons
ANS=B
Q47= The sterilization of glass-wares is done through
A : Fumigation
B : Disinfection
C : Dry heat
D : Moist heat
ANS- C
